Huckins C, Oakberg E F
Anat Rec. 1978 Dec;192(4):519-28. doi: 10.1002/ar.1091920406.
The spermatogonial populations in ten normal adult mice were analyzed using whole mounted seminiferous tubules. The undifferentiated A spermatogonia as well as the six generations of differentiating spermatogonia were clearly identifiable on whole mounts. Description plus quantitation of these cell types revealed that they behaved in essentially the same manner as their counterparts in the rat. Single undifferentiated A cells were classified as type As stem cell spermatogonia. They were distributed throughout the seminiferous epithelium, and by periodic mitoses, maintained their stock and furnished cells which would eventually differentiate. Although initially resembling the As spermatogonia, the progeny which were destined to differentiate were classified as type Aal spermatogonia because they were linked by cytoplasmic bridges, and because they usually underwent one or more synchronous mitotic divisions to form short chains of aligned cells. Ultimately, division of Aal cells were no longer seen, and the cells appeared to gradually acquire the typical morphological characteristics of A1 spermatogonia; these continued to differentiate according to the well-established pattern. It was concluded that the cyclic production of cohorts of A1 cells in this manner would ensure a continual supply of spermatogonia for differentiation.
使用整个生精小管对十只正常成年小鼠的精原细胞群体进行了分析。在整个标本上，可以清晰地识别出未分化的A型精原细胞以及六代正在分化的精原细胞。对这些细胞类型的描述和定量分析表明，它们的行为方式与大鼠中的对应细胞基本相同。单个未分化的A型细胞被归类为As干细胞精原细胞。它们分布在整个生精上皮中，并通过周期性有丝分裂维持其数量，并提供最终会分化的细胞。尽管最初类似于As精原细胞，但注定要分化的子代细胞被归类为Aal精原细胞，因为它们通过细胞质桥相连，并且因为它们通常进行一次或多次同步有丝分裂以形成排列整齐的短细胞链。最终，不再观察到Aal细胞的分裂，并且这些细胞似乎逐渐获得了A1精原细胞的典型形态特征；这些细胞继续按照既定模式进行分化。得出的结论是，以这种方式周期性产生A1细胞群体将确保持续供应精原细胞用于分化。
