鲤鱼（Cyprinus carpio L.）白细胞体外刺激的优化及动力学

Optimization and kinetics of in vitro stimulation of carp (Cyprinus carpio L.) leukocytes.

作者信息

Liewes E W, Van Dam R H, Vos-Maas M G, Bootsma R

出版信息

Vet Immunol Immunopathol. 1982 May;3(3):325-43. doi: 10.1016/0165-2427(82)90006-x.

Abstract

The optimization of a leukocyte stimulation microassay with carp (Cyprinus carpio L.) leukocytes is described. Leukocytes were isolated from the thymus, anterior kidney, spleen, mid-kidney and peripheral blood. Leukocyte cultures were stimulated with PHA-P, LPS (Escherichia coli 055: B5) PWM, ConA and PPD from Mycobacterium fortuitum. The optimum incubation temperature for leukocyte cultures differed 3.5 days for leukocyte cultures derived from lymphoid organs and 4.5 days for peripheral blood lymphocyte cultures. Leukocytes from various organ sources showed similar reactivity patterns to stimulation in vitro by different mitogens. The results of these mitogen stimulations did not present sufficient arguments in favour of compartmentation.

摘要

本文描述了用鲤鱼（Cyprinus carpio L.）白细胞优化白细胞刺激微量测定法的过程。白细胞从胸腺、前肾、脾脏、中肾和外周血中分离得到。白细胞培养物用PHA-P、LPS（大肠杆菌055:B5）、PWM、ConA和偶然分支杆菌的PPD进行刺激。淋巴细胞器官来源的白细胞培养物的最佳孵育温度为3.5天，外周血淋巴细胞培养物为4.5天。来自不同器官来源的白细胞对不同有丝分裂原的体外刺激表现出相似的反应模式。这些有丝分裂原刺激的结果没有提供足够的证据支持区室化。