Caspi R R, Shahrabani R, Kehati-Dan T, Avtalion R R
Dev Comp Immunol. 1984 Winter;8(1):61-70. doi: 10.1016/0145-305x(84)90010-7.
Peripheral blood leukocytes (PBL) of carp respond in vitro to a variety of phytomitogens, shown to be T-cell specific or B-cell specific in mammalian systems. Some basic differences have been observed in the proliferative response of carp PBL to PHA (phytohemagglutinin), ConA ( concanvalin A) and LPS (lipopolysaccharide): (1) The response to PHA and ConA was found to be highly dependent upon the continuous presence of mitogen in the medium, in contrast to LPS, where after the initial stimulation, cells could continue to proliferate for several days without mitogen. (2) Lymphoblasts grown in long term culture with either PHA or Con A could be transferred into medium containing the other mitogen without impairing cell proliferation, but cell growth was reduced to background level following transfer into LPS-containing medium. LPS grown cells continue to proliferate independently of the mitogen content of the medium. (3) Co-stimulation with LPS+PHA or LPS+ConA results in a synergistic response, while co-stimulation with PHA+ConA results in inhibition of DNA synthesis. (4) Several morphological differences have been observed between cells proliferating in the presence of PHA and those proliferating in the presence of LPS. It is suggested that while the PHA and ConA responsive cells may belong to the same lymphocyte subpopulation, they are distinct from the LPS-responsive subpopulation.
鲤鱼外周血白细胞（PBL）在体外对多种植物有丝分裂原产生反应，在哺乳动物系统中这些植物有丝分裂原已被证明是T细胞特异性或B细胞特异性的。在鲤鱼PBL对PHA（植物血凝素）、ConA（刀豆球蛋白A）和LPS（脂多糖）的增殖反应中观察到了一些基本差异：（1）发现对PHA和ConA的反应高度依赖于培养基中持续存在有丝分裂原，与之相反，对于LPS，在初始刺激后，细胞在没有有丝分裂原的情况下仍可继续增殖数天。（2）用PHA或Con A进行长期培养生长的淋巴母细胞可以转移到含有另一种有丝分裂原的培养基中而不损害细胞增殖，但转移到含LPS的培养基中后细胞生长降至背景水平。在LPS中生长的细胞继续独立于培养基中的有丝分裂原含量进行增殖。（3）LPS + PHA或LPS + ConA共同刺激会产生协同反应，而PHA + ConA共同刺激会抑制DNA合成。（4）在PHA存在下增殖的细胞与在LPS存在下增殖的细胞之间观察到了一些形态学差异。有人提出，虽然对PHA和ConA有反应的细胞可能属于同一淋巴细胞亚群，但它们与对LPS有反应的亚群不同。
