锥虫蓝及相关化合物对链球菌溶血素S产生及活性的影响。

Effects of trypan blue and related compounds on production and activity of streptolysin S.

作者信息

Taketo Y, Taketo A

出版信息

Z Naturforsch C Biosci. 1982 May-Jun;37(5-6):385-9. doi: 10.1515/znc-1982-5-607.

DOI:10.1515/znc-1982-5-607

Abstract

Most dyes related to trypan blue inhibited hemolytic activity of oligonucleotide-streptolysin S (SLS) complex, an exotoxin produced by Streptococcus pyogenes. Order of the inhibition was: trypan blue greater than benzo blue 2B greater than Congo red greater than Evans blue greater than benzo purpurine 4B greater than thiazine red greater than trypan red. When resting streptococcal cells were incubated with these dyes, significant amount of the hemolysin was produced. The carrier (or inducing) activity for SLS was further manifested in growing cell system and the potency of the compounds was as follows: Congo red greater than benzo blue 2B greater than trypan blue greater than Evans blue greater than Benzo purpurine 4B greater than or equal to thiazine red greater than trypan red. In this system, Congo red was more effective than oligonucleotide fraction rich in guanyl residue. Chromotrope 2B, H acid and o-tolidine were ineffective, as the carrier as well as the inhibitor. Based on these results, structure-function relationship among SLS, the carrier and the inhibitor was discussed.

摘要

大多数与锥虫蓝相关的染料可抑制寡核苷酸-链球菌溶血素S（SLS）复合物的溶血活性，该复合物是化脓性链球菌产生的一种外毒素。抑制顺序为：锥虫蓝＞苯并蓝2B＞刚果红＞伊文思蓝＞苯并紫嘌呤4B＞噻嗪红＞锥虫红。当静止的链球菌细胞与这些染料一起孵育时，会产生大量的溶血素。SLS的载体（或诱导）活性在生长细胞系统中进一步显现，这些化合物的效力如下：刚果红＞苯并蓝2B＞锥虫蓝＞伊文思蓝＞苯并紫嘌呤4B≥噻嗪红＞锥虫红。在该系统中，刚果红比富含鸟苷残基的寡核苷酸组分更有效。铬变素2B、H酸和邻联甲苯胺作为载体和抑制剂均无效。基于这些结果，讨论了SLS、载体和抑制剂之间的结构-功能关系。