链球菌溶血素S诱导的溶血：血红蛋白和86铷释放的动力学
Hemolysis induced by streptolysin S: kinetics of hemoglobin and 86Rubidium release.
作者信息
Hryniewicz W, Szmigielski S, Janiak M
出版信息
Zentralbl Bakteriol Orig A. 1977 Jun;238(2):201-7.
Hemolysis of human erythrocytes produced by streptolysin S (SLS) was investigated. Kinetic studies of hemoglobin (Hb) release exhibited typical for SLS latent phase with hemolysis 30-60 minutes after addition of the toxin. Hb release was preceded by efflux of 86Rubidium (86Rb) which started at 5th-15th minutes after addition of the toxin. In erythrocytes treated with 2 HU/ml of SLS about 100% of 86Rb was released after 15 minutes; no hemolysis was observed at this time. Incubation of blood cells in 0.3 M sucrose or 6% DMSO prevented SLS induced hemolysis. Trypan blue was also inhibitory. Bovine serum albumin acclerated 86Rb and Hb release. The possible mechanism of this phenomenon is discussed. The results obtained indicate that SLS-produced hemolysis of human erythrocytes is an osmotic process.
对链球菌溶血素S（SLS）所致人红细胞溶血进行了研究。血红蛋白（Hb）释放的动力学研究显示，SLS具有典型的潜伏期，在加入毒素后30 - 60分钟出现溶血。在加入毒素后第5 - 15分钟开始出现86铷（86Rb）外流，随后出现Hb释放。在用2 HU/ml SLS处理的红细胞中，15分钟后约100%的86Rb被释放；此时未观察到溶血。血细胞在0.3 M蔗糖或6%二甲基亚砜中孵育可防止SLS诱导的溶血。台盼蓝也具有抑制作用。牛血清白蛋白加速了86Rb和Hb的释放。讨论了这一现象的可能机制。所得结果表明，SLS所致人红细胞溶血是一个渗透过程。
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