Kirchick H J, Keyes P L, Frye B E
Acta Endocrinol (Copenh). 1982 Jun;100(2):266-73. doi: 10.1530/acta.0.1000266.
Immature alloxan-diabetic rats injected with pregnant mare's serum gonadotropin (PMSG) do not ovulate and the LH surge is absent. In the present studies we have examined the effects of several insulin treatments on the LH surge and ovulation in alloxan-diabetic rats. Rats were male diabetic by injection of alloxan on day 24 of age. Only those rats with fasting blood glucose concentrations exceeding 180 mg/100 ml on day 237 were considered diabetic. PMSG was injected on day 30. Rats received insulin either by injection (2, 3, 4 or 6 IU/100 g/day); Ultralente B.I.D.) or by subcutaneous implants (Alzet osmotic minipumps; 1.8 or 2.4 IU/day). None of the diabetic rats without insulin treatment ovulated. Some of the animals in each insulin treatment group ovulated, however, the percentage of animals ovulating was highly variable from experiment to experiment when the insulin was given by injection. When insulin was administered by osmotic minipump, the results were more consistent, with at least 60% of the rats ovulating in each experiment. LH surges were found on the afternoon of presumed pro-oestrus (day 32) in diabetic insulin-treated rats which ovulated. In confirmation of previous results, rats without insulin treatment did not have LH surges. Although the site of insulin action has not been determined, these data indicate that the LH surge mechanism in the immature PMSG-treated rat is insulin-dependent.
给未成熟的四氧嘧啶糖尿病大鼠注射孕马血清促性腺激素（PMSG）后，它们不会排卵且无促黄体生成素（LH）高峰。在本研究中，我们检测了几种胰岛素治疗方法对四氧嘧啶糖尿病大鼠LH高峰和排卵的影响。大鼠在24日龄时通过注射四氧嘧啶成为糖尿病大鼠。仅那些在237日龄时空腹血糖浓度超过180mg/100ml的大鼠被视为糖尿病大鼠。在30日龄时注射PMSG。大鼠通过注射（2、3、4或6IU/100g/天；长效胰岛素，每日两次）或通过皮下植入（Alzet渗透微型泵；1.8或2.4IU/天）接受胰岛素。未接受胰岛素治疗的糖尿病大鼠均未排卵。然而，每个胰岛素治疗组中的一些动物排卵了，但是当通过注射给予胰岛素时，每次实验中排卵动物的百分比差异很大。当通过渗透微型泵给予胰岛素时，结果更一致，每次实验中至少60%的大鼠排卵。在假定的发情前期下午（32日），在排卵的糖尿病胰岛素治疗大鼠中发现了LH高峰。正如之前的结果所证实的，未接受胰岛素治疗的大鼠没有LH高峰。尽管胰岛素作用的部位尚未确定，但这些数据表明，在未成熟的经PMSG治疗的大鼠中，LH高峰机制是胰岛素依赖性的。
