Kirchick H J, Keyes P L, Frye B E
Endocrinology. 1978 Jun;102(6):1867-73. doi: 10.1210/endo-102-6-1867.
The effects of alloxan-induced diabetes on ovulation and other ovarian responses were investigated in immature rats injected with PMS gonadotropin (PMSG, 15 IU/100 g) on day 30 of age. Rats were killed on day 32 (presumed proestrus) or on day 33, at which time the oviducts were examined for ova. Ovarian weight gain was similar in control and diabetic rats and Graafian follicles were present in both groups on day 32. None of the diabetic rats ovulated while 96% of the control rats ovulated. Anovulation in diabetic rats could not be attributed to a drug side-effect of alloxan or to a lack of ovarian responsiveness, as 90% of the animals ovulated after treatment with insulin or with hCG (5 IU). Measurements of serum estradiol and LH on the morning of presumed proestrus revealed that concentrations of these hormones were not different in control and diabetic rats. However, measurements of LH in blood samples taken in the afternoon from control rats showed an LH surge, whereas no LH surge was found in diabetic rats. Thus, anovulation in immature diabetic rats treated with PMSG is not caused by an attenuation of ovarian responsiveness or by decreased secretion of estradiol, but rather is due to the loss of the LH surge.
在30日龄时给未成熟大鼠注射孕马血清促性腺激素（PMSG，15 IU/100 g），研究四氧嘧啶诱导的糖尿病对排卵及其他卵巢反应的影响。大鼠于32日（假定为发情前期）或33日处死，此时检查输卵管中的卵子。对照组和糖尿病大鼠的卵巢增重相似，且两组在32日时均存在格拉夫卵泡。糖尿病大鼠均未排卵，而对照组大鼠的排卵率为96%。糖尿病大鼠不排卵并非由于四氧嘧啶的药物副作用或卵巢反应性缺乏，因为90%的动物在用胰岛素或人绒毛膜促性腺激素（hCG，5 IU）治疗后排卵。在假定的发情前期早晨测定血清雌二醇和促黄体生成素（LH），结果显示对照组和糖尿病大鼠中这些激素的浓度无差异。然而，下午采集的对照组大鼠血样中的LH测定显示有LH峰，而糖尿病大鼠中未发现LH峰。因此，用PMSG处理的未成熟糖尿病大鼠不排卵并非由卵巢反应性减弱或雌二醇分泌减少所致，而是由于LH峰缺失。
