Hough-Evans B R, Howard J
Biochim Biophys Acta. 1982 Jul 30;698(1):56-61. doi: 10.1016/0167-4781(82)90184-1.
Plasmodium falciparum DNA was prepared from cells cultured in vitro in human erythrocytes. The P. falciparum DNA was mixed with a tritium-labeled Escherichia coli DNA standard, and the kinetics of reassociation were measured using hydroxyapatite chromatography. It was found that the P. falciparum genome size is equal to 3.8 . 10(8) nucleotide pairs, and that a repetitive component is present which contains about 10% of the DNA. The average repetition frequency in this component is 95 copies of each sequence.
恶性疟原虫DNA是从在人红细胞中体外培养的细胞中制备的。将恶性疟原虫DNA与氚标记的大肠杆菌DNA标准品混合，并使用羟基磷灰石色谱法测量重缔合动力学。发现恶性疟原虫基因组大小等于3.8×10⁸个核苷酸对，并且存在一个重复组分，其包含约10%的DNA。该组分中的平均重复频率是每个序列95个拷贝。
