Morozova E N, Falina N N, Denisova N P, Barkova L V, Psurtseva N V
Biokhimiia. 1982 Jul;47(7):1181-5.
The degree of heterogeneity of the proteolytic complex from the fungus Flammulina velutipes was studied by gel chromatography and analytical isoelectrofocusing. The fibrinolytic, thrombolytic, caseinolytic, endo- and aminopeptidase activities of the enzyme complex were compared to those of Aspergillus terricola and Streptomyces griseus proteinases. The proteolytic complex under study consists of at least two proteinases with pI 6.1 and 7.1, which possess fibrinolytic, thrombolytic and endopeptidase activities and of two aminopeptidases with pI 5.5 and 6.05. All these activities are inhibited by metal-chelating reagents. A low caseinolytic activity of the complex suggests that it can successfully be employed as a therapeutic agent.
通过凝胶色谱法和分析等电聚焦法研究了金针菇真菌蛋白水解复合物的异质性程度。将该酶复合物的纤维蛋白溶解、血栓溶解、酪蛋白溶解、内切肽酶和氨肽酶活性与土曲霉和灰色链霉菌蛋白酶的活性进行了比较。所研究的蛋白水解复合物至少由两种蛋白酶组成，其等电点分别为6.1和7.1，具有纤维蛋白溶解、血栓溶解和内切肽酶活性，还有两种等电点分别为5.5和6.05的氨肽酶。所有这些活性均受金属螯合剂抑制。该复合物较低的酪蛋白溶解活性表明它可成功用作治疗剂。
