尿液中硫代硫酸盐的测定

Determination of thiosulphate in urine.

作者信息

Sörbo B, Ohman S

出版信息

Scand J Clin Lab Invest. 1978 Oct;38(6):521-7. doi: 10.1080/00365517809108814.

Abstract

A method is described for the determination of thiosulphate in urine. After removal of interfering compounds, including endogenous thiocyanate by ion exchange, thiosulphate is converted to thiocyanate in the presence of cyanide and cupric ions. The thiocyanate formed is concentrated by ion exchange, eluted with an acid solution of ferric ions and the ferric thiocyanate complex determined colorimetrically. Healthy human subjects excreted 31.7 +/- 12.8 mumol/24 h (mean +/- SD) thiosulphate.

摘要

本文描述了一种测定尿液中硫代硫酸盐的方法。通过离子交换去除包括内源性硫氰酸盐在内的干扰化合物后，硫代硫酸盐在氰化物和铜离子存在下转化为硫氰酸盐。形成的硫氰酸盐通过离子交换进行浓缩，用铁离子的酸性溶液洗脱，然后比色法测定硫氰酸铁络合物。健康人体受试者每24小时排泄31.7±12.8微摩尔硫代硫酸盐（平均值±标准差）。