Neudeck L D, Fournier D J
Lab Anim Sci. 1980 Apr;30(2 Pt 1):209-11.
A technique was described in which mouse kidneys were perfused with a silicone rubber compound. The technique involved cannulation of the left ventricle of the heart and subsequent perfusion of the entire systemic circulation of the mouse. Constant pressure was maintained to prevent distortion artifacts in the resultant renal microvascular cast. The method is adaptable to perfusion of other organs or animals.
描述了一种用硅橡胶化合物灌注小鼠肾脏的技术。该技术包括对小鼠心脏左心室进行插管,随后对小鼠的整个体循环进行灌注。维持恒定压力以防止所得肾微血管铸型中出现变形伪影。该方法适用于其他器官或动物的灌注。