Tseng S C, Lee P C, Ells P F, Bissell D M, Smuckler E A, Stern R
Hepatology. 1982 Jan-Feb;2(1):13-8. doi: 10.1002/hep.1840020104.
The cellular sources of collagen in normal rat liver have been examined. Hepatocytes and nonparenchymal (sinusoidal) cells were isolated and established in primary monolayer culture. These cells were incubated with radiolabeled proline in the presence of l-ascorbate and beta-aminopropionitrile. Nondialyzable material was prepared from the cell layer and the medium from each type of culture. The level of collagen accumulation was determined by measuring labeled hydroxyproline and sensitivity to purified bacterial collagenase. In hepatocytes, collagen represented 0.2% of both secreted and cell-associated labeled protein. In sinusoidal cells, collagen was 3.2% of secreted and 1.1% of cell-associated proteins. The total secreted labeled collagen, expressed per microgram of DNA, was similar in hepatocytes and sinusoidal cells. However cell-associated collagen in hepatocyte culture was approximately 10-fold that present in sinusoidal cells. These findings indicate that, while collagen formation is a relatively important function of sinusoidal cells, in normal liver the contribution of hepatocytes to total hepatic collagen accumulation may be substantial.
已对正常大鼠肝脏中胶原蛋白的细胞来源进行了研究。分离出肝细胞和非实质（窦状隙）细胞，并进行原代单层培养。将这些细胞在L-抗坏血酸和β-氨基丙腈存在的情况下与放射性标记的脯氨酸一起孵育。从每种培养类型的细胞层和培养基中制备不可透析物质。通过测量标记的羟脯氨酸和对纯化的细菌胶原酶的敏感性来确定胶原蛋白的积累水平。在肝细胞中，胶原蛋白占分泌型和细胞相关标记蛋白的0.2%。在窦状隙细胞中，胶原蛋白分别占分泌蛋白的3.2%和细胞相关蛋白的1.1%。以每微克DNA表示的总分泌标记胶原蛋白在肝细胞和窦状隙细胞中相似。然而，肝细胞培养物中细胞相关胶原蛋白约为窦状隙细胞中的10倍。这些发现表明，虽然胶原蛋白形成是窦状隙细胞的一个相对重要的功能，但在正常肝脏中，肝细胞对肝脏总胶原蛋白积累的贡献可能很大。
