Shiratori Y, Ichida T, Geerts A, Wisse E
Laboratory for Cell Biology and Histology, Faculty of Medicine and Pharmacy, Free University of Brussels, Belgium.
Dig Dis Sci. 1987 Nov;32(11):1281-9. doi: 10.1007/BF01296379.
In an attempt to elucidate the role of fat-storing cells (FSCs) in liver fibrosis, we investigated the collagen synthesis by FSCs freshly isolated from rats treated with CCl4, with vitamin A, and from untreated rats. FSCs from CCl4-treated rats contained a small number of lipid droplets and an abundant rough endoplasmic reticulum (RER), while those from vitamin A-treated rats showed numerous large lipid droplets and scanty RER. The population doubling times of FSCs isolated from normal, CCl4-treated, and vitamin A-treated rats were 38 +/- 4.3, 24 +/- 2.5, and 48 +/- 6.3 hr, respectively. The rate of collagen synthesis by FSCs from CCl4-treated rats was four- to sixfold enhanced, while collagen synthesis by FSCs from vitamin A-treated rats was suppressed. The ratio of collagen type I to type III produced by FSCs from CCl4 rats was enhanced as compared with control rats (94.7:5.3 vs 87.6:12.4). Therefore, FSCs can be considered to play an important role in the pathogenesis of liver fibrosis.
为了阐明贮脂细胞（FSCs）在肝纤维化中的作用，我们研究了从用四氯化碳（CCl4）处理的大鼠、用维生素A处理的大鼠以及未处理的大鼠中新鲜分离出的FSCs的胶原合成情况。来自CCl4处理大鼠的FSCs含有少量脂滴和丰富的粗面内质网（RER），而来自维生素A处理大鼠的FSCs则显示有大量大脂滴且RER稀少。从正常、CCl4处理和维生素A处理的大鼠中分离出的FSCs的群体倍增时间分别为38±4.3、24±2.5和48±6.3小时。来自CCl4处理大鼠的FSCs的胶原合成速率提高了4至6倍，而来自维生素A处理大鼠的FSCs的胶原合成受到抑制。与对照大鼠相比，来自CCl4处理大鼠的FSCs产生的I型胶原与III型胶原的比例有所增加（94.7:5.3对87.6:12.4）。因此，可以认为FSCs在肝纤维化的发病机制中起重要作用。
