Campwala H Q, Desforges J F
J Lab Clin Med. 1982 Jan;99(1):25-8.
The presence of bound heme pigment was compared in membranes of AA and SS cells. Spectophotometric study of membranes prepared by hypotonic lysis and washed free of hemoglobin revealed the presence of an irreversibly oxidized hemoglobin derivative, HCh. SS cell membranes contained significantly more HCh than did the AA cell membranes. When red cells were separated into cohorts of various density on a discontinuous density gradient of Stractan, washed membranes from the highest-density AA cells had significantly more HCh than did the remainder. In contrast, when density-separated SS cells were studied, no significant difference could be demonstrated in the HCh content of membranes from various cohorts.
对AA细胞和SS细胞的细胞膜中结合血红素色素的存在情况进行了比较。通过低渗裂解制备并洗涤去除血红蛋白的细胞膜的分光光度研究表明存在一种不可逆氧化的血红蛋白衍生物HCh。SS细胞膜中所含的HCh明显多于AA细胞膜。当红细胞在不连续的Stractan密度梯度上分离成不同密度的组群时，来自最高密度AA细胞的洗涤后细胞膜所含的HCh明显多于其余细胞。相比之下，当对密度分离的SS细胞进行研究时，不同组群细胞膜的HCh含量未显示出显著差异。
