Hoversland R C, Weitlauf H M
J Reprod Fertil. 1982 Jan;64(1):223-6. doi: 10.1530/jrf.0.0640223.
Mouse embryos collected before implantation were incubated in vitro for 24 h with fluid rinsed from the uteri of ovariectomized female mice injected with progesterone, oestradiol-17 beta + progesterone, oestradiol-17 beta + progesterone, or oestradiol-17 beta alone. Although none of the zonae was completely dissolved, those incubated in fluid from animals treated with oestradiol + progesterone were subsequently more soluble in sodium thiocyanate (NaSCN) than those incubated similarly in control buffer, indicating a sublytic change during the incubation with uterine washings. Zonae incubated in fluid from animals injected with either hormone alone did not undergo such a change.
收集未着床的小鼠胚胎，将其与从注射了孕酮、雌二醇-17β+孕酮、雌二醇-17β+孕酮或单独注射雌二醇-17β的去卵巢雌性小鼠子宫冲洗出的液体一起在体外培养24小时。虽然没有一个透明带完全溶解，但与在对照缓冲液中进行类似培养的胚胎相比，在接受雌二醇+孕酮处理的动物的液体中培养的胚胎随后在硫氰酸钠（NaSCN）中更易溶解，这表明在与子宫冲洗液培养期间发生了亚溶解变化。在单独注射任何一种激素的动物的液体中培养的透明带没有发生这种变化。
