Fishel S B
J Reprod Fertil. 1979 Jan;55(1):91-100. doi: 10.1530/jrf.0.0550091.
Uterine secretions were analysed after labelling with L-14,5-3H]leucine. During pro-oestrus major protein peaks were seen with approximate mol. wt 2.0 X 10(4), 2.7 X 10(4), 4.0 X 10(4), 5.7 X 10(4), 7.8 X 10(4) and 13.0 X 10(4). Qualitative changes were observed during the first 4 days of pregnancy. On day 4 p.c. secretions revealed two prominent peaks with mol. wt 6.7 X 10(4) and 12.5 X 10(4), four peaks between 2.0 and 4.7 X 10(4) and one at 8.5 X 10(4) and one at 20.0 X 10(4). Profiles similar to those on mimic Day 4 p.c., and implantation, were induced in ovariectomized females by administration of exogenous progesterone and oestradiol, whereas the proteins detected after administration of exogenous oestradiol alone were essentially similar to those found at pro-oestrus. Ovariectomized mice treated with the steroid sequence to mimic Day 4 p.c. profiles but not given the final injection of oestradiol showed no detectable labelled proteins. Injection of progesterone or oestradiol alone, in the final sequence of injections given to ovariectomized pregnant females treated with progesterone, did not induce blastocyst implantation. These studies indicate a synergistic relationship between progesterone and oestrogen in the secretion of uterine macromolecules.
用[L-14,5-³H]亮氨酸标记后，对子宫分泌物进行了分析。在动情前期可见主要的蛋白质峰，其分子量约为2.0×10⁴、2.7×10⁴、4.0×10⁴、5.7×10⁴、7.8×10⁴和13.0×10⁴。在妊娠的前4天观察到了定性变化。在妊娠第4天，分泌物显示出两个突出的峰，分子量分别为6.7×10⁴和12.5×10⁴，在2.0至4.7×10⁴之间有四个峰，一个在8.5×10⁴，一个在20.0×10⁴。通过给予外源性孕酮和雌二醇，在去卵巢的雌性动物中诱导出了与妊娠第4天模拟日和着床时相似的图谱，而单独给予外源性雌二醇后检测到的蛋白质与动情前期发现的蛋白质基本相似。用类固醇序列处理以模拟妊娠第4天图谱但未给予最后一次雌二醇注射的去卵巢小鼠未检测到标记蛋白质。在给予孕酮处理的去卵巢妊娠雌性动物的最后一次注射序列中，单独注射孕酮或雌二醇均未诱导胚泡着床。这些研究表明孕酮和雌激素在子宫大分子分泌中存在协同关系。
