Kara U A, Kindl H
Eur J Biochem. 1982 Jan;121(3):533-8. doi: 10.1111/j.1432-1033.1982.tb05819.x.
Protein bodies were prepared from cotyledons of germinating seeds of cucumber (Cucumis sativus) in different ways: the organelles either obtained from protoplasts by lysis or from cotyledons by mechanical disintegration were separated on sucrose-density gradients. In addition, a non-aqueous procedure was employed to isolate protein bodies. Marker proteins indicative of membranes of other organelles were carefully assayed. By this means contaminations in the purified protein-body fractions could be ruled out. Isolated protein bodies were separated into crystalloids, matrix, and membranes. The membranes were purified and characterized according to their equilibrium density (rho = 1.20 kg/l) on sucrose gradients by flotation or sedimentation. Protein-body membranes labelled in the phospholipid moiety were prepared and analyzed after application of [methyl-14C] choline or [32P] phosphate in vivo.
通过不同方法从黄瓜（Cucumis sativus）发芽种子的子叶中制备蛋白体：通过裂解从原生质体获得的细胞器或通过机械破碎从子叶获得的细胞器在蔗糖密度梯度上进行分离。此外，采用非水程序分离蛋白体。仔细测定了指示其他细胞器膜的标记蛋白。通过这种方式，可以排除纯化的蛋白体组分中的污染物。分离出的蛋白体被分为晶体、基质和膜。根据膜在蔗糖梯度上的平衡密度（ρ = 1.20 kg/l），通过浮选或沉降对膜进行纯化和表征。在体内应用[甲基 - 14C]胆碱或[32P]磷酸盐后，制备并分析了在磷脂部分标记的蛋白体膜。
