Sturm A, Schwennesen K, Kindl H
Eur J Biochem. 1985 Aug 1;150(3):461-8. doi: 10.1111/j.1432-1033.1985.tb09044.x.
Lipid bodies from fat-mobilizing cotyledons of cucumber and other Cucurbitaceae were investigated. Proteins and glycoproteins were analyzed by electrophoresis and then used to characterize the lipid body membrane at different stages of cell development. Contaminations by other membranes or organelles were ruled out by comparing the main constituents from the endoplasmic reticulum, cytosol, glyoxysomes and protein bodies with the pattern of the lipid body membrane, considering both the prevalent peptides and the dominating glycoproteins. Among the proteins of lipid body membranes in ripening and germinating cotyledons, a 90-kDa peptide was found as unique marker of lipid bodies at the stage of fat mobilization. The 90-kDa protein was purified, and antibodies against it were raised in rabbits. By means of immunoprecipitation and electrophoretic analysis it was demonstrated that the synthesis of the 90-kDa form located in lipid bodies shows a transient increase and subsequent decline, with maximal values being observed at day 3 of germination. At this stage, the rate of de novo synthesis was compared considering lipid body proteins and other organellar proteins. The 90-kDa protein appeared as the lipid body constituent that is synthesized and assembled in the organelle by far at the highest rates.
对黄瓜及其他葫芦科植物中负责脂肪动员的子叶中的脂质体进行了研究。通过电泳分析蛋白质和糖蛋白，然后用于表征细胞发育不同阶段的脂质体膜。通过比较内质网、细胞质、乙醛酸循环体和蛋白体的主要成分与脂质体膜的模式，排除了其他膜或细胞器的污染，同时考虑了普遍存在的肽和主要的糖蛋白。在成熟和发芽子叶的脂质体膜蛋白中，发现一种90 kDa的肽是脂肪动员阶段脂质体的独特标志物。纯化了90 kDa的蛋白质，并在兔子体内产生了针对它的抗体。通过免疫沉淀和电泳分析表明，位于脂质体中的90 kDa形式的合成呈现出短暂的增加然后下降，在发芽第3天观察到最大值。在此阶段，考虑脂质体蛋白和其他细胞器蛋白，比较了从头合成的速率。90 kDa的蛋白质是迄今为止在该细胞器中合成和组装速率最高的脂质体成分。
