Shapiro S Z, Murray M
Infect Immun. 1982 Feb;35(2):410-6. doi: 10.1128/iai.35.2.410-416.1982.
The humoral immune responses to Trypanosoma brucei infection were examined in N'dama and in Zebu, two breeds of cattle recognized for their differing susceptibility to trypanosomiasis. Regardless of the clinical course, animals of both breeds produced antibodies to nonsurface trypanosome antigen(s) detectable by both immunodiffusion and immune fluorescence. As a new approach to assessment of the humoral response to trypanosome infection, protein antigens responded to were isolated by immune precipitation, and their molecular weights were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This allowed the detection of differences in the immune response which correlated with the clinical course of the disease. All cattle of both breeds which exhibited a capacity to control the disease recognized at least one of three specific antigens: protein of 110,000, 150,000, and 300,000 daltons. The N'dama, which proved less susceptible to the disease, generally responded to more of the three identified trypanosome protein antigens than did the Zebu. Animals which died of trypanosomiasis failed to produce detectable antibodies to any of the three specific proteins, although they sometimes exhibited antibodies to another trypanosome antigen.
对锥虫病易感性不同的两个牛品种——恩达马牛和瘤牛，检测了它们对布氏锥虫感染的体液免疫反应。不论临床病程如何，两个品种的动物都产生了针对非表面锥虫抗原的抗体，这些抗体可通过免疫扩散和免疫荧光检测到。作为评估对锥虫感染的体液反应的一种新方法，通过免疫沉淀分离出了有反应的蛋白质抗原，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定了它们的分子量。这使得能够检测到与疾病临床病程相关的免疫反应差异。两个品种中所有表现出控制疾病能力的牛都识别出三种特定抗原中的至少一种：分子量为110,000、150,000和300,000道尔顿的蛋白质。事实证明对该病较不易感的恩达马牛，通常比瘤牛对三种已识别的锥虫蛋白质抗原中的更多抗原有反应。死于锥虫病的动物未能产生针对三种特定蛋白质中任何一种的可检测抗体，尽管它们有时会表现出针对另一种锥虫抗原的抗体。
