Purification of cytoplasmic aldehyde dehydrogenase by covalent chromatography on reduced thiopropyl-Sepharose 6B.
作者信息
Kitson T M
出版信息
J Chromatogr. 1982 Jan 1;234(1):181-6. doi: 10.1016/s0021-9673(00)81791-4.
PMID:7056826
Abstract
Disulfiram-modified cytoplasmic aldehyde dehydrogenase binds covalently to the thiol groups of reduced thiopropyl-Sepharose 6B under conditions in which mitochondrial aldehyde dehydrogenase does not bind. After washing the resin, the uncontaminated form of the enzyme is eluted by dithiothreitol solution.
摘要
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