O'Connor E F, Watson W H, Wyse G A
J Neurobiol. 1982 Jan;13(1):49-60. doi: 10.1002/neu.480130106.
The concentrations of various catecholamines in the nervous system of the horseshoe crab Limulus polyphemus have been determined by high-performance liquid chromatography with electrochemical detection. Dopamine, norepinephrine, epinephrine, and their precursor L-Dopa were present in appreciable quantities in discrete regions of the central nervous system and cardiac ganglion. The catecholamines were localized more precisely by use of the glyoxylic-acid-histofluorescence technique of de la Torre and Surgeon (1976). Catecholamine fluorescence appeared in protocerebral and tritocerebral neuropile, including regions of the central body and optic medulla. Posterior to these brain areas, tracts extended through the circumesophageal ganglionic ring and laterally out each of the pedal ganglia. Small clusters of large fluorescent somata were present in the protocerebrum. No fluorescence was observed in the corpora pedunculata.
利用高效液相色谱-电化学检测法测定了美洲鲎神经系统中各种儿茶酚胺的浓度。多巴胺、去甲肾上腺素、肾上腺素及其前体L-多巴在中枢神经系统和心脏神经节的离散区域中含量可观。通过使用德拉托雷和外科医生（1976年）的乙醛酸-组织荧光技术，对儿茶酚胺进行了更精确的定位。儿茶酚胺荧光出现在原脑和后脑神经纤维网中，包括中央体和视髓质区域。在这些脑区后方，神经束穿过食管周围神经节环并横向延伸至每个足神经节。原脑中存在小簇大型荧光细胞体。在柄状体内未观察到荧光。
