Dhoot G K, Perry S V
Muscle Nerve. 1982 Jan;5(1):39-47. doi: 10.1002/mus.880050108.
Using the immunoperoxidase technique, antibodies to the fast components of the troponin complex stained all regenerating cells after localized alcohol injury to rat skeletal muscle. Antibodies to slow troponin components stained only some of these cells. About 6 weeks after injury with the nerve intact, the fast and slow forms of the troponin components were located in different cells. During the later stages of regeneration, staining for myosin ATPase correlated with the staining with antibodies to fast and slow troponin components. A similar staining pattern was also observed in the early stages of regeneration of muscle denervated at the time of injury. In this case, antibodies to fast skeletal muscle troponin components continued to stain all the cells 10 weeks after injury. Injured denervated muscle cells stained equally dark by myosin ATPase after preincubation at pH 9.4 over this period. None of the regenerating myotubes in denervated muscle stained for myosin ATPase after preincubation at pH 4.3.
运用免疫过氧化物酶技术，针对肌钙蛋白复合体快速成分的抗体，在大鼠骨骼肌局部酒精损伤后能使所有再生细胞染色。针对慢速肌钙蛋白成分的抗体仅使其中部分细胞染色。在神经完好无损的情况下损伤约6周后，肌钙蛋白成分的快速和慢速形式位于不同细胞中。在再生后期，肌球蛋白ATP酶染色与针对快速和慢速肌钙蛋白成分抗体的染色相关。在损伤时去神经支配的肌肉再生早期也观察到类似的染色模式。在这种情况下，损伤10周后针对快速骨骼肌肌钙蛋白成分的抗体继续使所有细胞染色。在此期间，在pH 9.4预孵育后，去神经支配肌肉中的损伤细胞被肌球蛋白ATP酶染色的程度相同。在pH 4.3预孵育后，去神经支配肌肉中没有再生肌管被肌球蛋白ATP酶染色。
