HeLa S3细胞中的组蛋白以细胞周期阶段特异性的方式合成。

Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner.

作者信息

Marashi F, Baumbach L, Rickles R, Sierra F, Stein J L, Stein G S

出版信息

Science. 1982 Feb 5;215(4533):683-5. doi: 10.1126/science.7058333.

Abstract

The synthesis of histone proteins in G1 and S phase HeLa S3 cells was examined by two-dimensional electrophoretic fractionation of nuclear and total cellular proteins. Newly synthesized histones were detected only in S phase cells. Histone messenger RNA sequences, as detected by hybridization with cloned human histone genes, were present in the cytoplasm of S phase but not G1 cells.

摘要

通过对细胞核和全细胞蛋白质进行二维电泳分级分离，研究了G1期和S期HeLa S3细胞中组蛋白的合成情况。仅在S期细胞中检测到新合成的组蛋白。通过与克隆的人类组蛋白基因杂交检测到的组蛋白信使RNA序列，存在于S期细胞的细胞质中，而不存在于G1期细胞的细胞质中。