Melli M, Spinelli G, Arnold E
Cell. 1977 Sep;12(1):167-74. doi: 10.1016/0092-8674(77)90194-5.
Hybridization of HeLa cell RNA to the DNA of a recombinant phage containing sea urchin histone genes shows that histone mRNA of HeLa cells is synthesized throughout the entire cell cycle. Furthermore, histone messenger is synthesized in cells treated with cytosine arabinoside, an inhibitor of DNA duplication. Although histone RNA is found in the nucleus, the amount of cytoplasmic histone messenger is drastically reduced, in agreement with previous work. The absence of coupling between histone mRNA synthesis and DNA duplication shows that the regulatory process which determines the presence or absence of histone mRNA in the cytoplasm of HeLa cells is not at the transcriptional level.
将海拉细胞RNA与含有海胆组蛋白基因的重组噬菌体DNA进行杂交，结果表明海拉细胞的组蛋白mRNA在整个细胞周期中都有合成。此外，在经阿糖胞苷（一种DNA复制抑制剂）处理的细胞中也能合成组蛋白信使。尽管在细胞核中发现了组蛋白RNA，但细胞质中的组蛋白信使数量大幅减少，这与之前的研究结果一致。组蛋白mRNA合成与DNA复制之间不存在偶联，这表明决定海拉细胞细胞质中组蛋白mRNA有无的调节过程并非发生在转录水平。
