Nilsson I M, Rothman U, Stenberg P, Frohm B, Persson N H
Br J Haematol. 1982 Feb;50(2):335-43. doi: 10.1111/j.1365-2141.1982.tb01924.x.
A mucopolysaccharide was isolated from mussel broth. After sulphation, an electrophoretically homogenous product was obtained with a molecular weight of approximately 40 000 daltons and a sulphur content of about 12%. The sulphated polysaccharide (S-Lim) displayed an anticoagulant activity in a thrombin test system with human plasma. Unlike heparin, the anticoagulant effect of S-Lim was observed also in a thrombin-fibrinogen clotting system in the absence of AT III. Complete inhibition of the effect of 1.0 mg S-Lim was achieved with 1.5 mg protamine. In an activated partial thromboplastin time test system the anticoagulant activity of 1.0 mg S-Lim corresponded to about 40 iu of heparin. S-Lim was also found to inhibit thrombin-induced platelet aggregation. Furthermore, S-Lim inhibited the thrombin-dependent activation of factor XIII in human plasma. S-Lim did not affect various tests systems measuring factor Xa activity. It is concluded that this new sulphated mucopolysaccharide acts as a pure antithrombin with a potency corresponding to 40--50 iu heparin/mg S-Lim. In contrast to heparin and other heparinoids it does not require the presence of AT III.
从贻贝汤中分离出一种粘多糖。硫酸化后，得到一种电泳均一的产物，分子量约为40000道尔顿，硫含量约为12%。硫酸化多糖（S-Lim）在人血浆凝血酶测试系统中表现出抗凝活性。与肝素不同，在不存在抗凝血酶III（AT III）的凝血酶-纤维蛋白原凝血系统中也观察到了S-Lim的抗凝作用。1.5毫克鱼精蛋白可完全抑制1.0毫克S-Lim的作用。在活化部分凝血活酶时间测试系统中，1.0毫克S-Lim的抗凝活性相当于约40国际单位的肝素。还发现S-Lim可抑制凝血酶诱导的血小板聚集。此外，S-Lim可抑制人血浆中凝血酶依赖性的因子XIII激活。S-Lim不影响测量因子Xa活性的各种测试系统。得出的结论是，这种新的硫酸化粘多糖作为一种纯抗凝血酶起作用，其效力相当于40 - 50国际单位肝素/毫克S-Lim。与肝素和其他类肝素不同，它不需要AT III的存在。
