Membrane-active agents and heat-induced erythrocyte fragmentation.

Morphological observations of heated erythrocytes were made after preincubation with lysolecithin, diamide, p-chloromercuribenzoate, or N-ethyl maleimide and after ATP-depletion. Diamide (1.0 mM) reduced the critical temperature of fragmentation from 49 degrees C to 47 degrees C, and at higher concentrations the critical temperature was further reduced. The other sulphydryl reacting agents had little or no effect. Drug-induced spherocytes and ATP-depleted cells did not fragment. Membrane-active agents can effect the response of membranes to heat and the effect is dose-dependent.