Lewis E R, Cotman C W
Brain Res. 1982 Feb 4;233(1):29-44. doi: 10.1016/0006-8993(82)90927-1.
Embryonic septal tissues grafted alongside the hippocampal formation in neonatal rat hosts were used to test for the presence of competitive interactions between developing native axons and those from the septal grafts. When native septal afferents to the target hippocampal formation are removed at the time of implantation, axons from septal grafts visualized with acetylcholinesterase (AChE) histochemistry enter the appropriate laminar zones in the host hippocampus. The pattern of septal implant growth differs markedly when native septohippocampal fibers are left intact. Under these conditions, implant-associated AChE staining is intense in the cortical area surrounding the graft but little or no reaction product is present in the host dentate gyrus and hippocampus proper. A similar result is obtained when implant viability is enhanced by introducing it 3 days after the cavity is made. These data illustrate an apparent hierarchy in competitive interactions for developing septal fibers. Septal afferents can inhibit the growth of other septal fibers and commissural/associational (c/a) axons can exclude septal fibers from their terminal field. Septal fibers and entorhinal afferents will develop concurrently.
将胚胎期的隔区组织移植到新生大鼠宿主海马结构旁，用于检测发育中的宿主轴突与隔区移植轴突之间是否存在竞争性相互作用。当在植入时去除通向目标海马结构的宿主隔区传入纤维时，用乙酰胆碱酯酶（AChE）组织化学法显示的隔区移植轴突会进入宿主海马的相应分层区域。当宿主隔海马纤维保持完整时，隔区植入物的生长模式会明显不同。在这些条件下，植入物周围皮质区域的植入物相关AChE染色强烈，但宿主齿状回和海马本部几乎没有或没有反应产物。当在造腔3天后引入植入物以提高其活力时，也会得到类似的结果。这些数据说明了发育中的隔区纤维在竞争性相互作用中存在明显的等级关系。隔区传入纤维可以抑制其他隔区纤维的生长，联合/联合（c/a）轴突可以将隔区纤维排除在其终末场之外。隔区纤维和内嗅传入纤维将同时发育。
