Lewis E R, Mueller J C, Cotman C W
Brain Res Bull. 1980 May-Jun;5(3):217-21. doi: 10.1016/0361-9230(80)90160-4.
The technique of tissue implantation into neonatal rats was used to assess mechanisms underlying the development of afferent lamination in the rat hippocampal formation. Septal nuclei, dissected from the brains of rat embryos (E17-E20), were implanted into the occipital or entorhinal cortex of 2--3 day old neonates that simultaneously received fimbrial transections to remove hippocampal innervation by native septal efferents. As visualized with acetylcholinesterase (AChE) histochemistry, the distribution of enzyme activity in implant-innervated hippocampi is qualitatively identical to the pattern in unoperated animals. Fibers from septal implants placed into the entorhinal cortex, appear to interact with commissural/associational afferents and produce altered laminar zones in the dentate gyrus.
采用将组织植入新生大鼠的技术，来评估大鼠海马结构中传入纤维分层发育的潜在机制。从大鼠胚胎（E17 - E20）脑部分离出的隔核，被植入2 - 3日龄新生大鼠的枕叶或内嗅皮质，这些新生大鼠同时接受了穹窿横切术，以去除内源性隔传出纤维对海马的神经支配。用乙酰胆碱酯酶（AChE）组织化学方法观察到，植入物支配的海马中酶活性的分布在质量上与未手术动物的模式相同。植入内嗅皮质的隔核植入物发出的纤维，似乎与连合/联合传入纤维相互作用，并在齿状回中产生改变的分层区域。
