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纤细锥虫过氧化物酶体(微体)的精细结构与细胞化学

Fine structure and cytochemistry of peroxisomes (microbodies) Leptomonas samueli.

作者信息

Souto-Padrón T, de Souza W

出版信息

Cell Tissue Res. 1982;222(1):153-8. doi: 10.1007/BF00218295.

Abstract

Leptomonas samueli possesses in its cytoplasm a membrane-bounded organelle which can reach a length of 2.8 microns and a diameter of 0.2 microns. Catalase activity, which is inhibited by 3-amino-1, 2, 4-triazole, was detected at the ultrastructural level in the matrix of the organelle by using an alkaline diaminobenzidine medium. Freeze-fracture studies showed the presence of a large number of intramembranous particles on both the P and the E faces of the membrane of the organelle. Based on these data as well as on previous observations, it is suggested that the trypanosomatids possess an organelle that can be considered to be a peroxisome.

摘要

萨缪尔利细滴虫在其细胞质中拥有一个膜结合细胞器,其长度可达2.8微米,直径为0.2微米。通过使用碱性二氨基联苯胺培养基,在超微结构水平上于该细胞器的基质中检测到了被3-氨基-1,2,4-三唑抑制的过氧化氢酶活性。冷冻蚀刻研究表明,在该细胞器膜的P面和E面上均存在大量膜内颗粒。基于这些数据以及先前的观察结果,有人提出锥虫类拥有一种可被视为过氧化物酶体的细胞器。

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