人心脏细胞质天冬氨酸氨基转移酶的多种分子形式。
Multiple molecular forms of human heart cytoplasmic aspartate aminotransferase.
作者信息
Leung F Y, Henderson A R
出版信息
Clin Sci (Lond). 1982 Mar;62(3):337-9. doi: 10.1042/cs0620337.
PMID:7060339
Abstract
- Cytoplasmic aspartate aminotransferase was isolated and purified from human heart with a final specific activity of 236 units/mg of protein. 2. Three distinct peaks of aspartate aminotransferase activity were detected by isoelectric focusing with isoelectric points of 5.46, 5.60 and 5.71. Two minor subforms were also noted as shoulder patterns with pI 5.2 and 5.8. 3. These electrophoretic characteristics are similar to previous findings of multiple molecular forms detected in human liver and erythrocytes.
摘要
- 从人心脏中分离并纯化了细胞质天冬氨酸转氨酶，最终比活性为236单位/毫克蛋白质。2. 通过等电聚焦检测到天冬氨酸转氨酶活性的三个不同峰，其等电点分别为5.46、5.60和5.71。还注意到两个次要亚型，表现为等电点为5.2和5.8的肩峰模式。3. 这些电泳特征与先前在人肝脏和红细胞中检测到的多种分子形式的发现相似。
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