鸡卵转铁蛋白mRNA的完整核苷酸序列。

The complete nucleotide sequence of the chicken ovotransferrin mRNA.

作者信息

Jeltsch J M, Chambon P

出版信息

Eur J Biochem. 1982 Feb;122(2):291-5. doi: 10.1111/j.1432-1033.1982.tb05879.x.

DOI:10.1111/j.1432-1033.1982.tb05879.x

Abstract

The nucleotide sequence of an almost double-stranded cDNA copy [Cochet, M., Perrin, F., Gannon, F., Krust, A., Chambon, P., McKnight, G. S., Lee, D. C., Mayo, K. E., and Palmiter, R. D. (1979) Nucleic Acids Res. 6, 2435-2452] of chicken ovotransferrin (conalbumin) mRNA has been determined. Taking into account the previously reported 5'-end sequence [Cochet, M., Gannon, F., Hen, R., Maroteaux, L., Perrin, F., and Chambon, P. (1979) Nature (Lond.) 282, 567-574] we present the complete nucleotide sequence of the ovotransferrin mRNA from which the amino acid sequence of the protein is inferred. A computer and statistical analysis of the nucleotide sequence reveals a pattern of internal homology which confirms that the present-day chicken ovotransferrin gene (and by extrapolation the transferrin genes of other species) has evolved by duplication and gives some support to the quadruplication hypothesis of transferrin evolution.

摘要

已确定鸡卵转铁蛋白（伴清蛋白）mRNA的一个几乎双链cDNA拷贝[科谢特，M.，佩兰，F.，甘农，F.，克鲁斯特，A.，尚邦，P.，麦克奈特，G.S.，李，D.C.，梅奥，K.E.，帕尔米特，R.D.（1979年）《核酸研究》6，2435 - 2452]的核苷酸序列。考虑到先前报道的5′端序列[科谢特，M.，甘农，F.，亨，R.，马罗托，L.，佩兰，F.，尚邦，P.（1979年）《自然》（伦敦）282，567 - 574]，我们给出了卵转铁蛋白mRNA的完整核苷酸序列，并由此推断出该蛋白质的氨基酸序列。对核苷酸序列进行的计算机和统计学分析揭示了一种内部同源性模式，这证实了现今的鸡卵转铁蛋白基因（并由此推断其他物种的转铁蛋白基因）是通过复制进化而来的，并且为转铁蛋白进化的四倍体假说提供了一些支持。