Nara N, Bessho M, Hirashima K, Momo H
Exp Hematol. 1982 Jan;10(1):20-5.
Effects of chloramphenicol (CP) on hematopoietic inductive microenvironment (HIM) were studied using in vitro and in vivo assay systems. HIM was represented as fibroblast colonies (CFUF) in in vitro culture. CP suppressed the growth of not only granuloid committed progenitor cells (CFUC), but also CFUF in in vitro culture at the concentration of 10, 50 and 100 micrograms/ml. To analyze the function of HIM in vivo, the subcutaneous bone implantation method was used. The recovery of hematopoietic stem cells in subcutaneously implanted femora of mice, which were treated with a 500 mg/kg dose of CP daily for 6 days, was significantly decreased compared to the sham treated group. Suppressive effect of CP on HIM was shown. The important role of the derangement of HIM on the pathogenesis of CP-induced aplastic anemia was discussed.
使用体外和体内检测系统研究了氯霉素（CP）对造血诱导微环境（HIM）的影响。在体外培养中，HIM表现为成纤维细胞集落（CFUF）。在体外培养中，CP在浓度为10、50和100微克/毫升时，不仅抑制粒系定向祖细胞（CFUC）的生长，也抑制CFUF的生长。为了分析HIM在体内的功能，采用了皮下骨植入法。与假处理组相比，每天用500毫克/千克剂量的CP处理6天的小鼠皮下植入股骨中造血干细胞的恢复明显降低。显示了CP对HIM的抑制作用。讨论了HIM紊乱在CP诱导的再生障碍性贫血发病机制中的重要作用。
