Hotta T, Murate T, Utsumi M, Hirabayashi N, Yamada H
Exp Hematol. 1983 Feb;11(2):107-13.
The origin of spleen colony-forming units (CFUs) and fibroblastoid colony-forming cells (CFUF) repopulating the marrow of femurs subcutaneously implanted in syngeneic and semi-syngeneic mice was studied by making use of a chromosome marker for CFUs and a cytotoxicity test for CFUF. The CFUs present in implants during first 4 weeks were of a mixture of those from the donor and the host, and thereafter, were exclusively of host origin. On the other hand, the colony growth of CFUF from the marrow of C57BL/6 femurs grafted in B6CBAF1 mice remained almost totally unaffected by treatment with C57BL/6 anti-B6CBAF1 serum as late as 40 weeks after implantation. Quite similar results were obtained from the marrow-depleted femur implants. It is concluded from the present studies that chimeric marrow consisting of hemopoietic cells of host origin and stromal cells of donor origin can repopulate the femurs within a matter of several weeks after subcutaneous implantation.
利用针对脾集落形成单位（CFU）的染色体标记和针对成纤维细胞样集落形成细胞（CFUF）的细胞毒性试验，研究了在同基因和半同基因小鼠皮下植入的股骨骨髓中重新填充的脾集落形成单位（CFU）和成纤维细胞样集落形成细胞（CFUF）的起源。植入后前4周植入物中的CFU是供体和宿主来源的混合物，此后则完全是宿主来源。另一方面，在B6CBAF1小鼠中移植的C57BL/6股骨骨髓中的CFUF集落生长，直到植入后40周，用C57BL/6抗B6CBAF1血清处理后仍几乎完全不受影响。从骨髓耗尽的股骨植入物中也获得了非常相似的结果。从目前的研究得出结论，由宿主来源的造血细胞和供体来源的基质细胞组成的嵌合骨髓在皮下植入后的几周内可以重新填充股骨。
