Scott L D, Cahall D L
Gastroenterology. 1982 Apr;82(4):737-45.
This study was designed to define the role of the interdigestive myoelectric complex in small intestinal bacteriostasis. In rats, six monopolar electrodes were surgically sewn to the small intestine at equal intervals. One week later myoelectric activity was recorded. Under different experimental conditions, segments of duodenum and ileum were cultured quantitatively, both aerobically and anaerobically. Five groups of 6 electrode-equipped animals each were studied after an overnight fast: rats in which (a) the interdigestive myoelectric complex was present, (b) the interdigestive myoelectric complex was disrupted for 6 h using morphine sulfate, (c) the interdigestive myoelectric complex was disrupted for 15 h using morphine sulfate, (d) the interdigestive myoelectric complex was disrupted for 15 h using phenylephrine, and (e) the interdigestive myoelectric complex returned after 15 h of morphine sulfate effect. In control rats and during baseline records before drug administration in the other four groups, the interdigestive myoelectric complex was present. Activity fronts cycled at regular intervals in the proximal small intestine and moved aborally. Activity fronts disappeared following both morphine and phenylephrine, with varying degrees of inhibition of spike activity. Titers of microorganisms increased after 6 h, becoming statistically significant at 15 h; this effect was seen with both drugs. However, titers were similar to controls in groups 5. These results show that the interdigestive myoelectric complex is an important regulator at bacterial growth in the small intestine.
本研究旨在确定消化间期肌电复合波在小肠抑菌中的作用。在大鼠身上，将六个单极电极以相等间隔手术缝合至小肠。一周后记录肌电活动。在不同实验条件下，对十二指肠和回肠段进行需氧和厌氧定量培养。在禁食过夜后，对五组每组6只装有电极的动物进行研究：(a)存在消化间期肌电复合波的大鼠；(b)使用硫酸吗啡使消化间期肌电复合波中断6小时的大鼠；(c)使用硫酸吗啡使消化间期肌电复合波中断15小时的大鼠；(d)使用去氧肾上腺素使消化间期肌电复合波中断15小时的大鼠；(e)在硫酸吗啡作用15小时后消化间期肌电复合波恢复的大鼠。在对照大鼠以及其他四组给药前的基线记录期间，存在消化间期肌电复合波。活动波锋在近端小肠以规则间隔循环并向口外移动。吗啡和去氧肾上腺素均可使活动波锋消失，对锋电位活动有不同程度的抑制。微生物滴度在6小时后升高，在15小时时具有统计学意义；两种药物均出现这种效应。然而，第5组的滴度与对照组相似。这些结果表明，消化间期肌电复合波是小肠细菌生长的重要调节因子。
