Prakash R K, Schilperoort R A
J Bacteriol. 1982 Mar;149(3):1129-34. doi: 10.1128/jb.149.3.1129-1134.1982.
By use of the Southern blot hybridization technique the extent of DNA homology was determined between the Nif plasmid of a number of fast-growing Rhizobium species and Ti plasmids of the octopine (pTiAch5) and nopaline (pTiC58) type. DNA sequences common to these plasmids were located on functional maps of the Ti plasmids. No homology between Nif plasmids and the T region of Ti plasmids was detected.
利用Southern印迹杂交技术,确定了多种快速生长的根瘤菌属的固氮质粒与章鱼碱型(pTiAch5)和胭脂碱型(pTiC58)Ti质粒之间的DNA同源程度。这些质粒共有的DNA序列定位在Ti质粒的功能图谱上。未检测到固氮质粒与Ti质粒的T区域之间存在同源性。