通过DNA-DNA杂交检测根癌土壤杆菌中诱导肿瘤的质粒DNA序列。
Detection of tumor-inducing plasmid DNA sequence in Agrobacterium tumefaciens by DNA-DNA hybridization.
作者信息
Furukawa K, Hasunuma K, Hatanaka S, Hayashi T
出版信息
Radioisotopes. 1984 Aug;33(8):543-6. doi: 10.3769/radioisotopes.33.8_543.
Abstract
Absence of plasmid DNA sequences in non-tumorigenic (crown gall tumor) strains of Agrobacterium tumefaciens was confirmed by DNA-DNA hybridization between purified tumor-inducing (Ti) plasmid DNAs isolated from the progenitor tumorigenic strains and 3H-labeled whole cell DNAs from tumorigenic strains and their non-tumorigenic derivatives. The genes controlling tumorigenicity in plant and utilizability of nopaline as their sole nitrogen source were confirmed to locate on Ti-plasmids.
摘要
通过从致瘤性原始菌株中分离得到的纯化的致瘤（Ti）质粒DNA与来自致瘤性菌株及其非致瘤性衍生物的3H标记全细胞DNA之间的DNA-DNA杂交，证实了根癌土壤杆菌的非致瘤性（冠瘿瘤）菌株中不存在质粒DNA序列。控制植物致瘤性以及以胭脂碱作为唯一氮源的可利用性的基因被证实位于Ti质粒上。
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Detection of tumor-inducing plasmid DNA sequence in Agrobacterium tumefaciens by DNA-DNA hybridization.通过DNA-DNA杂交检测根癌土壤杆菌中诱导肿瘤的质粒DNA序列。
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