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用质粒DNA对豌豆根瘤菌进行遗传转化。

Genetic transformation of Rhizobium leguminosarum by plasmid DNA.

作者信息

Bullerjahn G S, Benzinger R H

出版信息

J Bacteriol. 1982 Apr;150(1):421-4. doi: 10.1128/jb.150.1.421-424.1982.

DOI:10.1128/jb.150.1.421-424.1982
PMID:7061403
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC220134/
Abstract

We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No colonies that were doubly drug resistant were recovered in parallel control experiments.

摘要

我们通过在含有R68.45质粒DNA和20 mM氯化镁的情况下冻融细胞悬浮液，证明了R68.45质粒DNA对豆科根瘤菌的遗传转化。对卡那霉素和四环素具有抗性的克隆以每个受体细胞10(-8)的频率回收。在平行对照实验中未回收双抗药性菌落。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2be1/220134/09bee8b21a07/jbacter00257-0431-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2be1/220134/09bee8b21a07/jbacter00257-0431-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2be1/220134/09bee8b21a07/jbacter00257-0431-a.jpg

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本文引用的文献

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GENETIC TRANSFORMATION OF RHIZOBIUM: A REVIEW OF THE WORK OF R. BALASSA.根瘤菌的遗传转化：R. 巴拉萨研究工作综述
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Genetic transformation of Rhizobium meliloti by plasmid DNA.用质粒DNA对苜蓿根瘤菌进行遗传转化。
Gene. 1981 Nov;15(2-3):279-83. doi: 10.1016/0378-1119(81)90137-2.
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Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.用于革兰氏阴性菌的广宿主范围DNA克隆系统：苜蓿根瘤菌基因文库的构建
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R factor transfer in Rhizobium leguminosarum.根瘤菌中R因子的转移
J Gen Microbiol. 1974 Sep;84(1):188-98. doi: 10.1099/00221287-84-1-188.
9
Transformation and physical properties of R-factor RP4 transferred from Escherichia coli to Rhizobium trifolii.R因子RP4从大肠杆菌转移至三叶草根瘤菌后的转化及物理特性
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10
A simple method for the preparation of large quantities of pure plasmid DNA.一种制备大量纯质粒DNA的简单方法。
Biochim Biophys Acta. 1975 Apr 2;383(4):457-63. doi: 10.1016/0005-2787(75)90318-4.