利用抗生素抗性突变体和营养缺陷型，在血清学不同的日本根瘤菌菌株内及菌株间进行质粒转移。
Plasmid transfer within and between serologically distinct strains of Rhizobium japonicum, using antibiotic resistance mutants and auxotrophs.
作者信息
Pilacinski W P, Schmidt E L
出版信息
J Bacteriol. 1981 Feb;145(2):1025-30. doi: 10.1128/jb.145.2.1025-1030.1981.
Abstract
Methionine-requiring and pantothenic acid-requiring auxotrophs of Rhizobium japanicum USDA 31, as well as highly antibiotic-resistant mutants of R. japonicum strains USDA 31, USDA 110, USDA 138, and Webster 48, were isolated. These mutants were used to transfer the P-1 group plasmids R68.45 and RP4 within and between strains USDA 31, USDA 110, and Webster 48. Attempts to demonstrate transfer of either plasmid to strain USDA 138 were unsuccessful.
摘要
分离出了日本根瘤菌USDA 31的蛋氨酸需求型和泛酸需求型营养缺陷型菌株，以及日本根瘤菌菌株USDA 31、USDA 110、USDA 138和韦伯斯特48的高抗药性突变体。这些突变体被用于在USDA 31、USDA 110和韦伯斯特48菌株内部以及它们之间转移P-1组质粒R68.45和RP4。将这两种质粒转移到USDA 138菌株的尝试均未成功。
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