Hoover R L, Klausner R, Karnovsky M J
J Biol Chem. 1982 Mar 10;257(5):2151-4.
We have previously reported that cis-unsaturated free fatty acids, e.g. linoleic acid, introduced as free fatty acids into lymphocyte membranes inhibited surface immunoglobulin capping. Trans-unsaturated and saturated free fatty acids had no effect. These results were interpreted as being due to perturbation of specific membrane lipid domains. Corps, A. N., Pozzan, T., Hesketh, T. R., and Metcalfe, J. C. (1980) J. Biol. Chem. 255, 10566-10568) in a recent paper argued, however, that linoleic acid causes depletion of ATP, as measured by the luciferin/luciferase assay, and respiratory uncoupling. We now present data showing that under the same conditions as the capping inhibition, there is neither ATP depletion, as found using a hexokinase/glucose-6-phosphate dehydrogenase assay, nor respiratory uncoupling. Linoleic acid produces artifacts in ATP measurements made using the luciferin/luciferase assay which leads to an erroneous conclusion regarding the ATP dependence of the capping inhibition.
我们之前曾报道，以游离脂肪酸形式引入淋巴细胞膜的顺式不饱和游离脂肪酸，如亚油酸，会抑制表面免疫球蛋白帽化。反式不饱和脂肪酸和饱和游离脂肪酸则没有这种作用。这些结果被解释为是由于特定膜脂结构域受到干扰所致。然而，科尔普斯、A.N.、波赞、T.、赫斯基思、T.R.和梅特卡夫、J.C.（1980年）在最近一篇论文中指出，通过荧光素/荧光素酶测定法测得，亚油酸会导致ATP耗竭以及呼吸解偶联。我们现在给出的数据表明，在与帽化抑制相同的条件下，使用己糖激酶/葡萄糖-6-磷酸脱氢酶测定法并未发现ATP耗竭，也没有呼吸解偶联现象。亚油酸在用荧光素/荧光素酶测定法进行ATP测量时会产生假象，这导致了关于帽化抑制对ATP依赖性的错误结论。
