Sekimizu K, Yokoi H, Natori S
J Biol Chem. 1982 Mar 25;257(6):2719-21.
Antibody raised against S-II, a stimulatory factor of RNA polymerase II from Ehrlich ascites tumor cells, inhibited accurate transcription from adenovirus 2 major late promoter in a HeLa cell lysate. Manganese prevented accurate transcription, but it was essential for expression of the stimulatory activity of S-II. The results indicate that protein(s) cross-reacting immunologically with S-II in a HeLa cell lysate is essential for accurate transcription of truncated DNA, but the stimulatory activity of this factor(s) is not necessary for accurate transcription of truncated DNA.
针对S-II（艾氏腹水瘤细胞中RNA聚合酶II的一种刺激因子）产生的抗体，在HeLa细胞裂解物中抑制了腺病毒2主要晚期启动子的精确转录。锰会阻止精确转录，但它对于S-II刺激活性的表达至关重要。结果表明，在HeLa细胞裂解物中与S-II发生免疫交叉反应的蛋白质对于截短DNA的精确转录至关重要，但该因子的刺激活性对于截短DNA的精确转录并非必需。
