Callewaert D M, Smeekens S P, Mahle N H
J Immunol Methods. 1982;49(1):25-37. doi: 10.1016/0022-1759(82)90363-5.
A distribution-free statistical procedure for estimating kinetic parameters for cellular cytotoxicity reactions is described. Estimates of cytotoxic activity obtained using this procedure are considerably more precise than percent cytotoxicity values, lytic unit values, and V max values determined by least-squares analysis of the same sets of experimental data. Application of this procedure to the study of natural cytotoxicity allows for precise quantitative comparisons of the lytic activity of different lymphocyte preparations for a given target cell line, of a single lymphocyte preparation for different target cell lines, or of a given lymphocyte and target cell combination under different experimental conditions. This procedure, which does not involve a more complex experimental protocol than other methods, should also allow for more accurate assessment of the relative cytotoxic activity of lymphocytes obtained from patient populations.
本文描述了一种用于估计细胞毒性反应动力学参数的无分布统计程序。使用该程序获得的细胞毒性活性估计值比通过对同一组实验数据进行最小二乘法分析确定的细胞毒性百分比值、裂解单位值和Vmax值要精确得多。将该程序应用于自然细胞毒性研究，可以对给定靶细胞系的不同淋巴细胞制剂的裂解活性、单个淋巴细胞制剂对不同靶细胞系的裂解活性，或不同实验条件下给定淋巴细胞与靶细胞组合的裂解活性进行精确的定量比较。该程序并不比其他方法涉及更复杂的实验方案，它还应该能够更准确地评估从患者群体中获得的淋巴细胞的相对细胞毒性活性。
