兔子宫颈和子宫体中酸性糖胺聚糖的研究（作者译）

[Studies on acid glycosaminoglycans in rabbit uterine cervix and body (author's transl)].

作者信息

Nakaoka Y, Itoh M, Hirakawa S, Watanabe H, Amano H

出版信息

Nihon Sanka Fujinka Gakkai Zasshi. 1982 Jan;34(1):96-100.

Abstract

We aimed to identified cervical ripening at term pregnancy by measuring acid glycosaminoglycans (aGAG) in nonpregnant and immediately postpartum rabbits cervix and body. aGAG was extracted from the uterine tissues by the modified method of K. Anno (1964) and determined by the carbazole method (Bitter, 1962). Components of aGAG were separated by two dimensional electrophoresis (0.1M pyridine-0.47M formic acid in the first and 0.1M calcium acetate buffer in the second) using acetate cellulose strips. aGAG were separated five spots that were identified hyaluronic acid, heparan sulfate, chondroitin sulfate A, dermatan sulfate and chondroitin sulfate C. These results were confirmed by method of hyaluronidase, chondroitinase AC and ABC digestion test.

摘要

我们旨在通过测量未怀孕和产后即刻兔子的子宫颈及子宫体中的酸性糖胺聚糖（aGAG）来确定足月妊娠时的宫颈成熟度。aGAG采用K. 安诺（1964年）的改良方法从子宫组织中提取，并通过咔唑法（比特，1962年）进行测定。使用醋酸纤维素条通过二维电泳（第一向为0.1M吡啶 - 0.47M甲酸，第二向为0.1M醋酸钙缓冲液）分离aGAG的成分。aGAG分离出五个斑点，分别鉴定为透明质酸、硫酸乙酰肝素、硫酸软骨素A、硫酸皮肤素和硫酸软骨素C。这些结果通过透明质酸酶、软骨素酶AC和ABC消化试验方法得到证实。