人体胃组织中酸性糖胺聚糖的研究。

A study of acidic glycosaminoglycans in human gastric tissue.

作者信息

Sekino T, Murata K, Saito Y, Tsubura K

出版信息

Digestion. 1977;16(1-2):28-39. doi: 10.1159/000198052.

DOI:10.1159/000198052

Abstract

The acidic glycosaminoglycans (AGAG) in normal portions of human gastric tissue were separated by electrophoresis in 3 buffer systems. Paper chromatographic separation of the constitutional disaccharide units by digestion of chondroitin sulfates (CS) with chondroitinase-ABC and chondroitinase-AC was carried out after fractionation of CS by ion-exchange resin column chromatography. Thin-layer chromatography of hexosamines and other biochemical analysis were also performed. The presence of hyaluronic acid in the gastric tissue was substantiated by the enzymatic susceptibility to streptomyces hyaluronidase. The results indicated that human gastric AGAG consisted of, in the order of amount, heparan sulfates, dermatan sulfate, hyaluronic acid, chondroitin-4-sulfate, chondroitin-6-sulfate and presumably oversulfated chondroitin sulfate.

摘要

采用3种缓冲体系通过电泳法分离人胃组织正常部分中的酸性糖胺聚糖（AGAG）。在用离子交换树脂柱色谱法对硫酸软骨素（CS）进行分级分离后，通过用软骨素酶ABC和软骨素酶AC消化硫酸软骨素对组成性二糖单位进行纸色谱分离。还进行了己糖胺的薄层色谱分析和其他生化分析。胃组织中透明质酸的存在通过对链霉菌透明质酸酶的酶敏感性得到证实。结果表明，人胃AGAG按含量顺序由硫酸乙酰肝素、硫酸皮肤素、透明质酸、硫酸软骨素-4、硫酸软骨素-6以及可能的过度硫酸化硫酸软骨素组成。