Beran M, Spitzer G, Verma D S
J Lab Clin Med. 1982 Feb;99(2):247-53.
The direct effect of testosterone and its synthetic analogs on the maintenance of human erythroid progenitor cells (BFU-E) and GM-CFC was studied in suspension cultures. To avoid interference by serum components, the experiments were conducted in serum-free media containing insulin, transferrin, selenium, BSA, and alpha-thioglycerol. When added at concentrations between 10(-7)M and 10(-9)M, testosterone improved survival of BFU-E and GM-CFC. Of the nine analogs tested, eight improved BFU-E maintenance in vitro: nandrolone norethandrolone, and oxymetholone were most active, whereas etiocholanolone had only marginal effects. The influence of these compounds on GM-CFC was less pronounced, with testosterone showing the highest activity. It is concluded that testosterone and some of the synthetic analogs tested exert their hemopoietic effect, at least partly, by affecting the maintenance of erythroid and granulocytic stem cells, directly by increasing their survival or proliferation or indirectly by increasing the input from multipotent stem cell pool, or by both mechanisms.
在悬浮培养中研究了睾酮及其合成类似物对人红系祖细胞（BFU-E）和GM-CFC维持的直接作用。为避免血清成分的干扰，实验在含有胰岛素、转铁蛋白、硒、牛血清白蛋白和α-硫甘油的无血清培养基中进行。当以10^(-7)M至10^(-9)M的浓度添加时，睾酮可提高BFU-E和GM-CFC的存活率。在所测试的九种类似物中，有八种可改善体外BFU-E的维持：诺龙、诺乙雄龙和羟甲烯龙活性最高，而本胆烷醇酮只有轻微作用。这些化合物对GM-CFC的影响不太明显，睾酮活性最高。结论是，睾酮和一些所测试的合成类似物至少部分地通过影响红系和粒系干细胞的维持发挥其造血作用，直接作用是增加它们的存活或增殖，或间接作用是增加多能干细胞池的输入，或通过两种机制。
