Mock E J, Frankel A I
J Endocrinol. 1982 Feb;92(2):231-6. doi: 10.1677/joe.0.0920231.
A rapid procedure was developed and validated for collection of testicular vein blood from rats. Samples were collected from both testes and the heart within 2-5 min of transfer of the rat to the anaesthesia jar. There was no difference in levels of testosterone in testicular vein serum between successive samples from the same testis or alternate samples from paired testes. Both hemicastration and sham-hemicastration significantly depressed levels of testosterone in testicular vein serum 4 h after surgery. Peripheral serum testosterone levels were significantly depressed after hemicastration but not sham-hemicastration at 4 h after surgery. By 24 h after surgery, testosterone in testicular vein serum was doubled in the hemicastrated rat, and was restored in the sham-hemicastrated rat, thus achieving normal peripheral testosterone levels in both groups. Similar results were observed in bilaterally denervated rats. These rapid changes could not be attributed to serum LH, which maintained steady levels during the course of these experiments. It was concluded that there are two components in the response to hemicastration: a non-specific response within 4 h to sham-surgery or any other disturbance to the animal, which depresses testosterone output sharply, and a specific response within 24 h to hemicastration, which doubles testicular output. It is the non-specific response after sham-surgery which has simulated and obfuscated the response to hemicastration in the male rat.
已开发并验证了一种从大鼠收集睾丸静脉血的快速程序。在将大鼠转移到麻醉罐后的2 - 5分钟内，从两个睾丸和心脏采集样本。来自同一睾丸的连续样本或成对睾丸的交替样本之间，睾丸静脉血清中的睾酮水平没有差异。半阉割和假半阉割在手术后4小时均显著降低了睾丸静脉血清中的睾酮水平。手术后4小时，半阉割后外周血清睾酮水平显著降低，但假半阉割后未降低。手术后24小时，半阉割大鼠睾丸静脉血清中的睾酮增加了一倍，假半阉割大鼠的睾酮恢复正常，从而使两组的外周睾酮水平均达到正常。在双侧去神经大鼠中也观察到了类似的结果。这些快速变化不能归因于血清促黄体生成素（LH），在这些实验过程中其水平保持稳定。得出的结论是，对半阉割的反应有两个组成部分：在4小时内对假手术或动物的任何其他干扰的非特异性反应，这会急剧降低睾酮输出；以及在24小时内对半阉割的特异性反应，这会使睾丸输出增加一倍。正是假手术后的非特异性反应模拟并混淆了雄性大鼠对半阉割的反应。
