Shelley R, Price J C, Jun H W, Cadwallader D E, Capomacchia A C
J Pharm Sci. 1982 Feb;71(2):262-4. doi: 10.1002/jps.2600710230.
A rapid, specific, and sensitive reversed-phase high-performance liquid chromatographic (HPLC) assay for the quantitative determination of all-trans-retinoic acid (I) or 13-cis-retinoic acid (II) in rat serum without extraction of lyophilization is described. Chromatographic separation from retinol, serum components, and retinol acetate standard was achieved on octadecylsilane-coated particles with acetonitrile-1% ammonium acetate as th eluent. Serum samples (100 microliter) containing as little as 10 ng of retinoid were analyzed. Serum level profiles of rats dosed with the retinoids demonstrated the utility of the assay and indicated elimination half-lives of 0.58 and 0.92 hr for I and II, respectively.
本文描述了一种快速、特异且灵敏的反相高效液相色谱（HPLC）分析法，用于定量测定大鼠血清中的全反式维甲酸（I）或13-顺式维甲酸（II），无需冻干提取。在十八烷基硅烷键合硅胶颗粒上，以乙腈-1%醋酸铵为流动相，实现了与视黄醇、血清成分和醋酸视黄酯标准品的色谱分离。分析了含有低至10 ng类维生素A的血清样品（100微升）。用类维生素A给药的大鼠血清水平图谱证明了该分析方法的实用性，并表明I和II的消除半衰期分别为0.58小时和0.92小时。
