Brestkin A P, Viaz'menskaia M M, Dembo M A, Maĭzel' E B, Raĭkher I I
Vopr Med Khim. 1978 Sep-Oct;24(5):608-12.
A modified method is described for isolation of acetylcholinesterase from human erythrocytes using an additional step of gel filtration on Sephadex G-75. Preparations of acetylcholinesterase were liberated from thromaboplastic activity and their specific activity was increased due to removal of low molecular proteins and of the products of destruction of hemoglobin. Content of A and B isoantigens in the preparations obtained was rather low and content of hemoglobin, combined with other proteins in the form of oxyhemoglobin, did not exceed 12% of the total protein.
描述了一种改进方法，用于从人红细胞中分离乙酰胆碱酯酶，该方法使用了在葡聚糖凝胶G-75上进行凝胶过滤的额外步骤。乙酰胆碱酯酶制剂从凝血活性中释放出来，由于去除了低分子蛋白质和血红蛋白破坏产物，其比活性增加。所得制剂中A和B同种抗原的含量相当低，以氧合血红蛋白形式与其他蛋白质结合的血红蛋白含量不超过总蛋白的12%。
