Reactivation of DNA synthesis in aging diploid human skin fibroblasts by fusion with mouse L karyoplasts cytoplasts and whole L cells.

Diploid human skin fibroblasts derived from an 82-year-old donor with a 21-28 cell population doubling (CPD) range (where 28 CPD marked the end of the in vitro life span of the cells) were fused with whole L cells, L karyoplasts and L cytoplasts. The proportion of human nuclei incorporating tritiated thymidine after fusion was measured autoradiographically. Statistically significant increases in the labeling indices were found in the human nuclei in hybrid, heterodikaryon and cybrid cells when compared to control unfused human cells. Fusion of human diploid fibroblasts with human cytoplast derived from cells of the same CPD showed no significant changes in the labeling indices of the human nuclei.