Kolesnichenko T S, Medvinskiĭ A L
Ontogenez. 1982;13(1):83-7.
The formation and development of organotypic structure of the embryonic lung tissue was studied upon the cultivation of a suspension of dissociated cells of the lungs of 8-11 days old chick embryos as a hanging drop. The successive developmental stages of organotypic structures are discussed: 1) passive sedimentation of dissociated cells on the "bottom" of hanging drop; 2) redistribution of sedimented cells on the tension surface of the drop with the formation of multiple cell aggregations (centers of aggregation) connected by monolayer mesenchyme constrictions; 3) formation of a primary aggregate as a flat celled structure; 4) formation of a space organotypic aggregate with the structure characteristic for the initial lung tissue.
通过将8至11日龄鸡胚肺脏解离细胞悬液作为悬滴培养，研究了胚胎肺组织器官样结构的形成与发育。文中讨论了器官样结构的连续发育阶段：1）解离细胞在悬滴“底部”的被动沉降；2）沉降细胞在液滴张力表面重新分布，形成由单层间充质收缩连接的多个细胞聚集体（聚集中心）；3）形成初级聚集体，为扁平细胞结构；4）形成具有初始肺组织特征结构的空间器官样聚集体。
