Obregón M J
Ann Clin Biochem. 1982 Jan;19(Pt 1):29-34. doi: 10.1177/000456328201900107.
A highly sensitive radioimmunoassay for the determination of thyroxine in blood spotted on filter paper is described. The radioimmunoassay developed by Weeke and Orskov for determination of serum thyroxine was simplified and shortened, and its cost was decreased, for use in programmes for mass screening of thyroid function. The method permits the use of small (2 mm diameter) dried blood dots, thus avoiding interference by binding proteins. Polyethylene glycol 6000 is used for the final separation of antibody bound labelled thyroxine, instead of the expensive and time-consuming wick chromatography used in the reference method. Serum thyroxine concentrations obtained using dried blood by the modified procedure correlate well with those obtained using serum by the reference method. The distribution of serum thyroxine concentration for a population of 2134 babies is given.
本文描述了一种用于测定滤纸上血斑中甲状腺素的高灵敏度放射免疫分析方法。对Weeke和Orskov开发的用于测定血清甲状腺素的放射免疫分析方法进行了简化和缩短，并降低了成本，以用于甲状腺功能大规模筛查项目。该方法允许使用小的（直径2毫米）干血点，从而避免结合蛋白的干扰。聚乙二醇6000用于最终分离与抗体结合的标记甲状腺素，取代了参考方法中昂贵且耗时的灯芯色谱法。使用改良程序通过干血获得的血清甲状腺素浓度与通过参考方法使用血清获得的浓度相关性良好。给出了2134名婴儿群体的血清甲状腺素浓度分布情况。
